研究業績

主な原著論文

  • Motomura K, Tsuchi H, Komojiri M, Matsumoto A, Sugi N, Susaki D, Takeda A, Kinoshita T & *Maruyama D. Microtubules ensure transport of vegetative nuclei and sperm cells by fine-tuning their home positions. bioRxiv. (2024) DOI: 10.1101/2024.01.31.578224
  • *Sugi N, Susaki D, Mizuta Y, Kinoshita T & Maruyama D.　　Blue light irradiation induces pollen tube rupture in various flowering plants.　Plant Cell Physiol., 65 (5), 704-707, (2024) DOI:10.1093/pcp/pcae018
  • Sugi, N Izumi R, Tomomi S, Susaki D, Kinoshita T & * Maruyama D. Removal of the endoplasma membrane upon sperm cell activation after pollen tube discharge. Front. Plant Sci., 14, 1116289. (2023) DOI:10.3389/fpls.2023.1116289
  • Motomura K, Sugi N, Takeda, A, Yamaoka S & *Maruyama D. Possible molecular mechanisms of persistent pollen tube growth without de novo transcription. Front. Plant Sci., 13, 1020306. (2022) DOI:10.3389/fpls.2022.1020306.
  • Susaki D, Suzuki T, Maruyama D, Ueda M, Higashiyama T, Kurihara D. Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis. PLoS Biol. Mar 26;19(3):e3001123. doi: 10.1371/journal.pbio.3001123. （2021）
  • Motomura K, Takeuchi H, Notaguchi M, Tsuchi H, Takeda A, Kinoshita T, Higashiyama T, Maruyama D. Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex. Nat Commun. Apr 22;12(1):2331. doi: 10.1038/s41467-021-22661-8. (2021)
  • Ali MF, Fatema U, Peng X, Hacker SW, Maruyama D, Sun MX, Kawashima T. ARP2/3-independent WAVE/SCAR pathway and class XI myosin control sperm nuclear migration in flowering plants. Proc Natl Acad Sci U S A. Dec 22;117(51):32757-32763. doi: 10.1073/pnas.2015550117. (2020)
  • Goto C, Tamura K, Nishimaki S, Maruyama D, Hara-Nishimura I. The nuclear envelope protein KAKU4 determines the migration order of the vegetative nucleus and sperm cells in pollen tubes. J Exp Bot. Oct 22;71(20):6273-6281. doi: 10.1093/jxb/eraa367. (2020)
  • Nishikawa SI, Yamaguchi Y, Suzuki C, Yabe A, Sato Y, Kurihara D, Sato Y, Susaki D, Higashiyama T, Maruyama D. Arabidopsis GEX1 Is a Nuclear Membrane Protein of Gametes Required for Nuclear Fusion During Reproduction. Front Plant Sci. 2020 Oct 12;11:548032. doi: 10.3389/fpls.2020.548032. (2020)
  • Maruyama D, Higashiyama T, Endo T, and Nishikawa S, Fertilization-Coupled Sperm Nuclear Fusion is Required for Normal Endosperm Nuclear Proliferation. Plant Cell Physiol. 61: 29-40. doi: 10.1093/pcp/pcz158 (2019)
  • Motomura K, Kawashima T, Berger F, Kinoshita T, Higashiyama T, *Maruyama D., A Pharmacological Study of Arabidopsis Cell Fusion Between the Persistent Synergid and Endosperm.  Journal of Cell Science 131(2) jcs204123 (2018)
  • Susaki D, Maruyama D, Yelagandula R, Berger F, Kawashima T. Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana. Methods in molecular biology (Clifton, N.J.), 1669:47-54. DOI:10.1007/978-1-4939-7286-9_4 (2017)
  • Zhao X, Bramsiepe J, Van Durme M, Komaki S, Prusicki MA, Maruyama D, Forner J, Medzihradszky A, Wijnker E, Harashima H, Lu Y, Schmidt A, Guthörl D, Logroño RS, Guan Y, Pochon G, Grossniklaus U, Laux T, Higashiyama T, Lohmann JU, Nowack MK, Schnittger A. RETINOBLASTOMA RELATED1 mediates germline entry in Arabidopsis. Science (New York, N.Y.), 356:eaaf6532. DOI:10.1126/science.aaf6532 (2017)
  • Ohtsu M, Sato Y, Kurihara D, Suzaki T, Kawaguchi M, Maruyama D, Higashiyama T. Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines. Protoplasma, 254:2107-2115. DOI:10.1007/s00709-017-1105-0 (2017)
  • Ohtsu M, Kurihara D, Sato Y, Suzaki T, Kawaguchi M, Maruyama D, Higashiyama T. Fluorescent Labeling of the Cyst Nematode Heterodera glycines in Deep-Tissue Live Imaging.  Cytologia, 81:251-259. DOI:10.1508/cytologia.82.251 (2017)
  • Maruyama D, Higashiyama T,The end of temptation: the elimination of persistent synergid cell identity, Current opinion in plant biology, 34 122 – 126 (2016)
  • Kasahara RD, Notaguchi M, Nagahara S, Suzuki T, Susaki D, Honma Y, Maruyama D, Higashiyama T, Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization. Science advances, 2(10) e1600554(2016)
  • Maruyama D, Ohtsu M, Higashiyama T , Cell fusion and nuclear fusion in plants. Seminars in cell & developmental biology 60 127 – 135 (2016)
  • Motomura K, Berger F, Kawashima T, Kinoshita T, Higashiyama T, *Maruyama D. Fertilization-independent cell-fusion between the synergid and central cell in the polycomb mutant. Cell StrucCell Struc. Funct. 41:121-125 (2016) DOI: 10.1247/csf.16010
  • Maruyama D, Endo T, *Nishikawa S, BiP3 supports the early stages of female gametogenesis in the absence of BiP1 and BiP2 in Arabidopsis thaliana. Plant Signal. Behav. 10:e1035853 (2015) DOI: 10.1080/15592324.2015.1035853
  • *Maruyama D, Völz R, Takeuchi H, Mori T, Igawa T, Kurihara D, Kawashima T, Ueda M, Ito M, Umeda M, Nishikawa S, Groß-Hardt R, Higashiyama T, Rapid elimination of the persistent synergid through a cell fusion mechanism. Cell 161:907-918 (2015) DOI: 10.1016/j.cell.2015.03.018
  • Maruyama D, Yamamoto M, Endo T, *Nishikawa S, Different sets of ER-resident J-proteins regulate distinct polar nuclear-membrane fusion events in Arabidopsis thaliana. Plant Cell Physiol. 55:1937-1944 (2014) DOI: 10.1093/pcp/pcu120
  • Maruyama D, Sugiyama T, Endo T, *Nishikawa S, Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness. Plant Cell Physiol. 55:801-810 (2014) DOI: 10.1093/pcp/pcu018
  • Maruyama D, Hamamura Y, Takeuchi H, Susaki D, Nishimaki M, Kurihara D, Kasahara RD, *Higashiyama T, Independent control by each female gamete prevents the attraction of multiple pollen tubes. Dev. Cell 25:317-323 (2013) DOI: 10.1016/j.devcel.2013.03.013
  • Maruyama D, Endo T, *Nishikawa S, BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana. Proc. Nat. Acad. Sci. USA 107:1684-1689 (2010) DOI: 10.1073/pnas.0905795107
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その他の原著論文

  • Zhang, Y., Maruyama, D., Toda, E., Kinoshita, A., Okamoto, T., Mitsuda, N., Takasaki, H. & *Takagi, M. O. Transcriptome analyses uncover reliance of endosperm gene expression on Arabidopsis embryonic development. FEBS Lett.,597 (3), 407–417. (2023) DOI:10.1002/1873-3468.14570
  • Takasaki, H., Ikeda, M., Hasegawa, R., Yilin, Z., Sakamoto, S., Maruyama, D., Mitsuda, N., Kinoshita, T. & *Ohme-Takagi, M. Elongation of siliques without pollination 3 regulates nutrient flow necessary for embryogenesis. Plant Cell Physiol., 64 (1), 117–123. (2022) DOI:10.1093/pcp/pcac151
  • Susaki, D., Izumi, R., Oi, T., Takeuchi, H., Shin, J.M., Sugi, N., Kinoshita, T., Higashiyama, T., Kawashima, T. & *Maruyama, D.　F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells. Plant Cell, 35 (4) 1222–1240. (2022) DOI:10.1093/plcell/koac371
  • Susaki D, Suzuki T, Maruyama D, Ueda M, Higashiyama T & *Kurihara D. Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis. PLoS Biol. 19(3):e3001123. （2021） DOI: 10.1371/journal.pbio.3001123.
  • Ali MF, Fatema U, Peng X, Hacker SW, Maruyama D, Sun MX & *Kawashima T. ARP2/3-independent WAVE/SCAR pathway and class XI myosin control sperm nuclear migration in flowering plants. Proc. Natl. Acad. Sci. USA. 117(51):32757–32763. (2020) DOI: 10.1073/pnas.2015550117.
  • Goto C, Tamura K, Nishimaki S, Maruyama D & *Hara-Nishimura I. The nuclear envelope protein KAKU4 determines the migration order of the vegetative nucleus and sperm cells in pollen tubes. J. Exp. Bot.  71(20):6273–6281. (2020) DOI: 10.1093/jxb/eraa367.
  • Susaki D, Maruyama D, Yelagandula R, Berger F & *Kawashima T. Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana. Methods in molecular biology (Clifton, N.J.), 1669:47–54. (2017) DOI:10.1007/978-1-4939-7286-9_4
  • Zhao X, Bramsiepe J, Van Durme M, Komaki S, Prusicki MA, Maruyama D, Forner J, Medzihradszky A, Wijnker E, Harashima H, Lu Y, Schmidt A, Guthörl D, Logroño RS, Guan Y, Pochon G, Grossniklaus U, Laux T, Higashiyama T, Lohmann JU, Nowack MK & *Schnittger A. RETINOBLASTOMA RELATED1 mediates germline entry in Arabidopsis. Science (New York, N.Y.), 356:eaaf6532. (2017) DOI:10.1126/science.aaf6532
  • *Ohtsu M, Sato Y, Kurihara D, Suzaki T, Kawaguchi M, Maruyama D & Higashiyama T. Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines. Protoplasma, 254:2107–2115. (2017) DOI:10.1007/s00709-017-1105-0
  • *Ohtsu M, Kurihara D, Sato Y, Suzaki T, Kawaguchi M, Maruyama D & Higashiyama T. Fluorescent Labeling of the Cyst Nematode Heterodera glycines in Deep-Tissue Live Imaging.  Cytologia, 81:251–259. (2017) DOI:10.1508/cytologia.82.251
  • *Kasahara RD, Notaguchi M, Nagahara S, Suzuki T, Susaki D, Honma Y, Maruyama D & Higashiyama T, Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization. Sci. Adv., 2(10) e1600554 (2016) DOI:10.1126/sciadv.1600554
  • *Kasahara RD, Notaguchi M, Nagahara S, Suzuki T, Susaki D, Honma Y, Maruyama D, Higashiyama T, Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization. Sci. Adv. 2:e1600554 (2016)DOI: 10.1126/sciadv.1600554
  • Mizukami AG, Inatsugi R, Jiao J, Kotake T, Kuwata K, Ootani K, Okuda S, Sankaranarayanan S, Sato Y, Maruyama D, Iwai H, Garénaux E, Sato C, Kitajima K, Tsumuraya Y, Mori H, Yamaguchi J, Itami K, Sasaki N, *Higashiyama T, The AMOR arabinogalactan sugar chain induces pollen-tube competency to respond to ovular guidance. Curr. Biol. 26:1091-1097 (2016)DOI: 10.1016/j.cub.2016.02.040
  • Kawashima T, Maruyama D, Shagirov M, Li J, Hamamura Y, Yelagandula R, Toyama Y, *Berger F, Dynamic F-actin movement is essential for fertilization in Arabidopsis thaliana. eLIFE 3:e04501 (2014)DOI: 10.7554/eLife.04501
  • *Kasahara RD, Maruyama D, Higashiyama T, Fertilization recovery system is dependent on the number of pollen grains for efficient reproduction in plants. Plant Signal. Behav. 8:e23690 (2013)DOI: 10.4161/psb.23690
  • Kasahara RD, Maruyama D, Hamamura Y, Sakakibara T, Twell D, *Higashiyama T, Fertilization recovery after defective sperm cell release in Arabidopsis. Curr. Biol. 22:1084-1089 (2012)DOI: 10.1016/j.cub.2012.03.069
  • Yamamoto M, Maruyama D, Endo T, Nishikawa S, Arabidopsis thaliana has a set of J proteins in the endoplasmic reticulum that are conserved from yeast to animals and plants. Plant Cell Physiol. 49:1547-1562 (2008)DOI: 10.1093/pcp/pcn119
  • Nishikawa S, Zinkl GM, Swanson RJ, Maruyama D, *Preuss D, Callose (β-1, 3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth. BMC Plant Biol. 5:22 (2005)DOI: 10.1186/1471-2229-5-22
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英文総説

  • Sugi N & Maruyama D.   Exploring novel polytubey reproduction pathways utilizing cumulative genetic tools.　Plant Cell Physiol., 64 (5), 454–460. DOI:10.1093/pcp/pcad021 (2023)
  • Maruyama D, *Higashiyama T, The end of temptation: the elimination of persistent synergid cell identity. Curr. Opin. Plant Biol. 34:122-126 (2016)DOI: 10.1016/j.pbi.2016.10.011
  • *Maruyama D, Ohtsu M, Higashiyama T, Cell fusion and nuclear fusion in plants. Sem. in Cell Dev. Biol. In Press (2016)DOI: 10.1016/j.semcdb.2016.07.024
  • *Maruyama D, Kawashima T, Higashiyama T, Selective nuclear elimination in multinucleate cells. Oncotarget 6:30447-30448 (2015)DOI: 10.18632/oncotarget.5450
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著書総説等

  • 杉 直也, *丸山大輔  「内部形質膜から見る重複受精」 月刊 細胞  54(6) 32-35 2022年5月（2022）
  • *丸山大輔, 東山哲也　「新奇の植物細胞融合による花粉管の誘引停止機構」細胞工学 30:1164-1165 (2015)
  • *丸山大輔, 東山哲也　「経時観察でまだまだ見つかる新現象」日経バイオテク (2015) https://bio.nikkeibp.co.jp/article/news/20150730/186582/
  • *丸山大輔, 東山哲也　「植物の受精における細胞融合による残存助細胞の迅速な排除」ライフサイエンス　新着論文レビュー (2015)
    DOI: 10.7875/first.author.2015.051 (http://first.lifesciencedb.jp/archives/10061)
  • *丸山大輔, 東山哲也「花粉管の誘引停止の制御機構と受精戦略」化学と生物 52:380-386 (2014)
    DOI: doi.org/10.1271/kagakutoseibutsu.52.380
  • *丸山大輔, 東山哲也「2つの雌性生殖細胞による独立の制御が複数の花粉管の誘引を阻止する」ライフサイエンス　新着論文レビュー (2013)
    DOI: 10.7875/first.author.2013.070 (http://first.lifesciencedb.jp/archives/7195)
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受賞歴

  • 2018年9月　日本植物学会　奨励賞
  • 2016年4月19日 文部科学大臣表彰 若手科学者賞
  • 2015年11月17日 名古屋大学石田賞
  • 2015年9月5日 日本植物形態学会平瀬賞
  • 2015年8月27日 第２７回高遠シンポジウム 最優秀ポスター賞
  • 2015年7月1日 第６７回日本細胞生物学会大会 若手最優秀発表賞

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